bacteria

Purpose:
How concentration can affect the performance rate of disinfectant in killing micro-organisms.

Equipment:
3 agar plates., Slurry containing microorganisms, Savlon, Mark pen (permanent) Ruler, spotting tile, cello-tape Bunson burner and pipette

Concentration:
One control-water
100%
50%
10%

Hypothesis:
i think the greater the concentration the greater the clear zone will be. in that sense that the more savlon the more bacteria stopped from growing.

Variables:

• The independent variable concentration of savlon
• dependent variable clear zone (mm)
• controlled variable same size agar plate, the same type of disinfectant and same size filter paper  

possible factors for error:

• Agar plates gets contaminated 
• agar jelly cracks
• amount of yoghurt used 
• not sterilizing the tweezers

Method:
1.
Gather all your equipment.
2.
using a marker pen on the jelly-side label the section for the different dilutions and label what each section is for.
3.
cover the agar plate with diluted yoghurt.
4.
dilute the savlon to 100%, 50% and 10% .
5.
 using hole punch cut out 12 circles 4 for each plate.
6.
with sterilized tweezers dip your circle in each savlon dilute in order of 10,50 then 100 and place in and run your tweezers through the bunsen burner in between each circle different sections of the plate.
7.
once done tape the plate shut and place upside down.

Concerntration (%)	clearzone (mm) p1	clearzone (mm) p2	clearzone (mm) p3	Average
0%	0	0	0	0
10%	13	18	20	17
50%	22	25	25	24
100%	28	29	35	30.66666667

Conclusion:
After analyzing our results I have concluded that as savlon consecrations increases the clear zone also increases. Savlon has a active ingredient which is chlorhexidine this active ingredient is a bacterio static and disinfectant. a bacteria growth starts with a parent cell the parent cell replicates its own DNA then the parent cell starts to grow/Elongate. then the parent cell divides into two cell and crates 2 daughter cells which the daughter cell is an exact replica of the parent cell. This is happening because the cell is a-sexual. the salvon dose not kills the bacteria but stops the reproduction of the cell so it cannot make daughter cells, therefore, the colony of bacteria can not grow and then they die off.
the structure of a bacteria induces.

• flagellum
• plasma membrane
• capsule 
• cell wall
• chromosome
• nucleoid
• ribosome
• cytoplasm
• Pili

Evaluation:
yes, it worked but there were some interesting variables to certain clear zones. in some things, the results tuned out wrong and different from the rest. No question was raised because the results came out as expected .

Resources:
work I used before in class and google images